Comparison of gene expression differences between Caenorhabditis elegans grown in liquid culture versus on plates

ABSTRACT: The two major methods for growing C. elegans are liquid and plate culture. Differences in oxygenation and other environmental variables could affect many experimentally relevant biological processes, such as metabolism, cellular structure, and gene regulation. Worms on solid support move with deliberate sinusoidal movements, while worms in liquid thrash rapidly and almost constantly, often appearing elongated. We investigated differential gene expression under these two commonly used growth conditions. Embryos were isolated from adults grown on plates. After hatching, animals were placed either on plates or in liquid under standard conditions, and grown until young adulthood, approximately 48 hours later. RNA was isolated independently from each growth, differentially labeled with a fluo

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Christina Whittle 

PROVIDER: E-GEOD-21526 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress