Investigating the epigenetic effects of a prototype smoke-derived carcinogen in human cells

ABSTRACT: Global loss of DNA methylation and locus/gene-specific gain of DNA methylation are two distinct hallmarks of carcinogenesis. Aberrant DNA methylation is implicated in smoking-related lung cancer. In this study, we have comprehensively investigated the modulation of DNA methylation consequent to chronic exposure to a prototype smoke-derived carcinogen, benzo[a]pyrene diol epoxide (B[a]PDE), in genomic regions of significance in lung cancer, in normal human cells. We have used a pulldown assay for enrichment of the CpG methylated fraction of cellular DNA combined with microarray platforms, followed by extensive validation through conventional bisulfite-based analysis. Here, we demonstrate strikingly similar patterns of DNA methylation in non-transformed B[a]PDE-treated cells vs control using

ORGANISM(S): Homo sapiens

SUBMITTER: Xiwei Wu 

PROVIDER: E-GEOD-21532 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress