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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from Arabidopsis thaliana treated with NO donor SNP, compared to NO depletion by cPTIO

ABSTRACT: Nitric oxide (NO) is considered a key regulator of plant developmental processes and defense. Characterization of root-related phenotypes and the involvement of NO in the regulation of primary root growth. Three-experimental condition, SDW, SNP and cPTIO. Biological replicates: 3 control replicates (SDW) , 3 replicates treated with sodium nitroprusside (SNP), 3 replicates treated with 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO)

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Maria FernandezMarcos

PROVIDER: E-GEOD-21556 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Nitric Oxide Enhances Resistance to PEG-induced Water Deficiency is Associated with the Primary Photosynthesis Reaction in Triticum aestivum L.

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Transcription profiling of Arabidopsis treated with Trichostatin A (TSA) to inhibit of histone deacetylase in Arabiodopsis thaliana

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Transcription profiling of Arabidopsis seed germination in presence and absence of histone deaceatylase inhibitor, Trichostain A (TSA).

Project description:Histone acetylation is involved in the regulation of gene expression in plants and eukaryotes. Histone deacetylases (HDACs) are enzymes that catalyze the removal of acetyl groups from histones, which is associated with the repression of gene expression. To study the role of histone acetylation in the regulation of gene expression during seed germination, trichostatin A (TSA), a specific inhibitor of histone deacetylase, was used to treat imbibing Arabidopsis thaliana seeds. GeneChip arrays were used to show that TSA induces up-regulation of 45 genes and down-regulation of 27 genes during seed germination. Eight TSA-up-regulated genes were selected for further analysis - RAB18, RD29B, ATEM1, HSP70 and four late embryogenesis abundant protein genes (LEA). A gene expression time course shows that these eight genes are expressed at high levels in the dry seed and repressed upon seed imbibition at an exponential rate. In the presence of TSA, the onset of repression of the eight genes is not affected but the final level of repressed expression is elevated. Chromatin immunoprecipitation and HDAC assays show that there is a transient histone deacetylation event during seed germination at one day after imbibition, which serves as a key developmental signal that affects the repression of the eight genes. Experiment Overall Design: Three samples: Unimbibed seeds, untreated imbibed seeds, TSA-treated imbibed seeds.

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