Expression profiling of ligand-stimulated wild type and tamoxifen-resistant MCF-7

ABSTRACT: Quantitative phosphoproteome and transcriptome analysis of ligand-stimulated MCF-7 human breast cancer cells was performed to understand the mechanisms of tamoxifen resistance at a systems level. Phosphoproteome data revealed that wild type (WT) cells were more enriched with phospho-proteins than tamoxifen-resistant (TamR) cells after stimulation with ligands. Surprisingly, decreased phosphorylation after ligand perturbation was more common than increased phosphorylation. In particular, 17beta-estradiol (E2) induced down-regulation in WT cells at a very high rate. E2 and the ErbB ligand, heregulin (HRG) induced almost equal numbers of up-regulated phospho-proteins in WT cells. Pathway and motif activity analyses using transcriptome data additionally suggested that deregulated activation of

ORGANISM(S): Homo sapiens

SUBMITTER: Mariko Okada 

PROVIDER: E-GEOD-21618 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress