Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Highly Parallel SNP Genotyping Reveals High-resolution Landscape of Mono-allelic Ube3a Expression Associated with Locus-wide Antisense Transcription

ABSTRACT: Genomic imprinting is a mammalian-specific gene expression regulation system that distinguishes two parental alleles and yields parent-origin-specific gene expression. It has been identified approximately 90 imprinted gene loci in the mouse genome thus far. One of the molecular bases that establish genomic imprinting is through endogenous antisense transcription. In several imprinted loci, antisense transcription is observed in a repressive allele, probably contributing to the parent-origin-specific gene expression establishment. We investigated the allele- and strand-specific transcriptional dynamics of a megabase-wide genomic region of mouse Ube3a (ubiquitin protein ligase E3A), which is maternally expressed in a tissue-specific manner, by means of a highly parallel SNP genotyping platform that targets the tissue transcriptome. We successfully observed higher resolution transcriptional activity in the vicinity, including brain-specific widespread antisense transcription. We have listed up SNP sites within Ube3a-Snurf/Snrpn region between C57BL/6J and MSM/Ms. SNP sites were loaded onto Illumina GoldenGate Assay platform, and were assayed by targeting total RNA came from brain and liver of F1 hybrid mice.

ORGANISM(S): Mus musculus

SUBMITTER: Koji Numata

PROVIDER: E-GEOD-21667 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Highly Parallel SNP Genotyping Reveals High-resolution Landscape of Mono-allelic Ube3a Expression Associated with Locus-wide Antisense Transcription

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