ATAC and Mediator coactivators form a stable complex and regulates a set of non-coding RNA genes

ABSTRACT: The ATAC histone acetyl-transferase (HAT) and the Mediator coactivator complexes regulate independent and distinct steps during transcription initiation and elongation. Here we report the identification of a new stable molecular assembly formed between the ATAC and the Mediator complex in mouse embryonic stem cells. Moreover, we identify LUZP1 as a subunit of this meta coactivator complex (MECO). Finally, we demonstrate that MECO regulates a subset of RNA polymerase II transcribed non-coding RNA genes. Our findings establish that transcription coactivator complexes can form stable sub-complexes in order to facilitate their combined actions on specific target genes. For ChIP-seq analysis, 300µg of chromatin (DNA) was incubated with GST (mock), LUZP1 and GCN5 antibodies. Retrieved purified D

ORGANISM(S): Mus musculus

SUBMITTER: Laszlo Tora 

PROVIDER: E-GEOD-21717 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress