Mapping origins of replication in Arabidopsis thaliana

ABSTRACT: Genomic integrity requires faithful chromosome duplication. Origins of replication are the genomic sites where DNA replication initiates in every cell cycle. There are multiple origins scattered throughout the eukaryotic genome whose genome-wide identification has been a hard challenge, especially in multicellular organisms. Thus, very little is known on the distinctive features of origins in terms of DNA sequence and chromatin context at a genomic scale. Here we have profiled origins in Arabidopsis thaliana by high-throughput sequencing of purified nascent DNA strands. We have identified 1543 replication origins, which were uniformly distributed across the Arabidopsis genome and enriched in binding signals of two replication initiation proteins, CDC6 and ORC1. We have also analyzed novel

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Hume Stroud 

PROVIDER: E-GEOD-21828 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress