Comparison of murine colonic mucosal DNA between postnatal day 90 C57BL/6 wild type (WT) and their toll-like receptor 2 knockout (Tlr2-/-) counterparts by methylation specific amplification microarray (MSAM), and whole genomic gene expression profiling

ABSTRACT: BACKGROUND & AIMS: Toll-like receptor 2 (Tlr2) is important in bacterial pattern recognition and has been recognized as a modifier of intestinal inflammation. In this study we sought to determine the epigenomic, transcriptomic and microbiomic consequences of Tlr2 deficiency in the colonic mucosa of mice to gain insights into biological pathways that shape the interface between the gut microflora and the mammalian host. METHODS: Colonic mucosa from C57BL/6 and Tlr2-/- mice was interrogated by methylation specific amplification microarray (MSAM) to screen for changes in DNA methylation, with bisulfite pyrosequencing validation. Transcriptomic changes in the same tissue were analyzed by microarray expression profiling and real time RT-PCR. The mucosal microbiome was studied by high throughput

ORGANISM(S): Mus musculus

SUBMITTER: Richard Kellermayer 

PROVIDER: E-GEOD-21845 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress