Metabolism of a plant derived galactose-containing polysaccharide by Bifidobacterium breve UCC2003

ABSTRACT: In this study, we describe the functional characterisation of the B. breve UCC2003 gal locus, which is dedicated to the utilisation of galactan, a plant-derived polysaccharide. Using a combination of molecular approaches we conclude that the galA gene of B. breve UCC2003 encodes a beta-1,4-endogalactanase producing galacto-oligosaccharides, which are specifically internalised by an ABC transport system, encoded by galBCDE, and which are then hydrolysed to galactose moieties by a dedicated intracellular beta-galactosidase, specified by galG. The generated galactose molecules are presumed to be fed into the fructose-6-phosphate phosphoketolase pathway via the Leloir pathway, thereby allowing B. breve UCC2003 to use galactan as its sole carbon and energy source. In addition to these findings

ORGANISM(S): Bifidobacterium breve UCC2003

SUBMITTER: Mary O'Connell Motherway 

PROVIDER: E-GEOD-21865 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress