A direct role for cohesin in genome-wide gene regulation and Ecdysone-response in Drosophila salivary gland

ABSTRACT: Developmental abnormalities observed in Cornelia de Lange Syndrome (CdLS) have been genetically linked to mutations in the cohesin machinery. These findings raise the possibility that cohesin, in addition to its canonical function of mediating sister chromatid cohesion, might also be involved in regulating gene expression. We report that cleavage of cohesin’s kleisin subunit in post-mitotic Drosophila salivary glands induces major changes (both up and down) in the transcript levels of many genes. Kinetic analyses of changes in transcript levels upon cohesin cleavage reveal that a subset of genes responds to cohesin cleavage within a few hours. In addition, cohesin binds to most of these loci, suggesting that cohesin is directly regulating their expression. Amongst these genes are several t

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Bas van Steensel 

PROVIDER: E-GEOD-21874 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress