Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Sheep dendritic cells CD26+ and CD26- versus total dendritic cells

ABSTRACT: Transcriptomic profiling of ovine skin lymph dendritic cell subsets CD26+ and CD26- compared to total enriched lymph dendritic cells. CD26+ and CD26- dendritic cells (DC) from sheep skin lymph were sorted by flow cytometry and were analysed for their transcriptomic profile. We demonstrate that the minor sheep CD26+ skin lymph DC subset shares significant transcriptomic similarities with mouse CD8a+ and human BDCA3+ DC. This allowed the identification of a common set of phenotypic characteristics for CD8a-like DC in the 3 mammalian species, i.e. SIRPlo, CADM1hi, CLEC9Ahi, DEC205hi, XCR1hi. Compared to CD26- DC, the sheep CD26+ DC show (1) potent stimulation of allogeneic naive CD8+ T cells with high selective induction of the Ifn-gamma and Il22 genes; (2) dominant efficacy in activating specific CD8+ T cells against exogenous soluble antigen; (3) selective expression of functional pathways associated to high capacity for antigen cross-presentation. Reference design (total enriched lymph dendritic cells) - Biological replicates: 2 sheep #10081 and #30066

ORGANISM(S): Ovis aries

SUBMITTER: Doulaye Dembele 

PROVIDER: E-GEOD-21889 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Existence of CD8α-like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species.

Contreras Vanessa V   Urien Céline C   Guiton Rachel R   Alexandre Yannick Y   Vu Manh Thien-Phong TP   Andrieu Thibault T   Crozat Karine K   Jouneau Luc L   Bertho Nicolas N   Epardaud Mathieu M   Hope Jayne J   Savina Ariel A   Amigorena Sebastian S   Bonneau Michel M   Dalod Marc M   Schwartz-Cornil Isabelle I  

Journal of immunology (Baltimore, Md. : 1950) 20100811 6

The mouse lymphoid organ-resident CD8alpha(+) dendritic cell (DC) subset is specialized in Ag presentation to CD8(+) T cells. Recent evidence shows that mouse nonlymphoid tissue CD103(+) DCs and human blood DC Ag 3(+) DCs share similarities with CD8alpha(+) DCs. We address here whether the organization of DC subsets is conserved across mammals in terms of gene expression signatures, phenotypic characteristics, and functional specialization, independently of the tissue of origin. We study the DC  ...[more]

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