Tat RNA silencing suppressor activity contributes to perturbation of lymphocyte miRNAs by HIV-1

ABSTRACT: Herein expression trends of host miRNA were measured in CEMx174 lymphocytes infected with HIV-1NL4-3 or derivative strains deficient in Tat RSS activity (RSS) or vif-/vpr (VV), or unexposed to virus. As expected from previous studies, miRNA trends were different in naive and HIV-1 infected cells. Moreover, miRNA expression trends were similar for HIV-1 and vif-/vpr-deficient HIV-1, but diverged for RSS. Rather than generalized changes in miRNA levels, HIV-1 bearing the Tat K51A RSS mutation changed the steady state of a subset of miRNAs. The results are attributable to reduction in miRNA stability or change in miRNAs activity that altered steady state expression. Comparison of the miRNA expression trends in patient PBMC and the CEMx174 lymphocytes determined >50% overlap with a subset

ORGANISM(S): Homo sapiens

SUBMITTER: Amy Hayes 

PROVIDER: E-GEOD-21892 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress