Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Propagation of Adipogenic Signals through an Epigenomic Transition State

ABSTRACT: The transcriptional mechanisms by which temporary exposure to developmental signals instigates adipocyte differentiation are unknown. During early adipogenesis, we find transient enrichment of the glucocorticoid receptor (GR), CCAAT/enhancer binding protein b (CEBPb), p300, mediator subunit 1, and histone H3 acetylation near genes involved in cell proliferation, development and differentiation, including the gene encoding the master regulator of adipocyte differentiation, peroxisome proliferator activated receptor g2 (PPARg2). Occupancy and enhancer function are triggered by adipogenic signals, and diminish upon their removal. GR, which is required for adipogenesis but need not be active in the mature adipocyte, transiently functions with other enhancer proteins to propagate a new program of gene expression that includes induction of PPARg2, thereby providing a memory of the earlier adipogenic signal. Thus, the conversion of preadipocyte to adipocytes involves the formation of an epigenomic transition state that is not observed in cells at the beginning or end of the differentiation process. Genomic occupancy profiled by high throughput sequencing (ChIP-seq) from 3T3-L1 cells during differentiation for H3K9Ac, CEBPb and GR.

ORGANISM(S): Mus musculus

SUBMITTER: David Steger

PROVIDER: E-GEOD-21898 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Propagation of adipogenic signals through an epigenomic transition state.

Steger David J DJ Grant Gregory R GR Schupp Michael M Tomaru Takuya T Lefterova Martina I MI Schug Jonathan J Manduchi Elisabetta E Stoeckert Christian J CJ Lazar Mitchell A MA

Genes & development 20100501 10

The transcriptional mechanisms by which temporary exposure to developmental signals instigates adipocyte differentiation are unknown. During early adipogenesis, we find transient enrichment of the glucocorticoid receptor (GR), CCAAT/enhancer-binding protein beta (CEBPbeta), p300, mediator subunit 1, and histone H3 acetylation near genes involved in cell proliferation, development, and differentiation, including the gene encoding the master regulator of adipocyte differentiation, peroxisome proli ...[more]

PMID: 20478996

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Dataset Information

Propagation of Adipogenic Signals through an Epigenomic Transition State

Publications

Propagation of adipogenic signals through an epigenomic transition state.

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