Gene expression profile of the SVZ of the postnatal mouse brain

ABSTRACT: To determine gene expression profiles of neurogenesis, neural stem cell and notch signaling elements in the adult subventricular zone (SVZ) of the WT and CNP-hEGFR mouse, we used SuperArray limited gene array analysis. GEArrayTM expression array systems (cat#OMM404 SuperArray, Bethesda, MD) consisted of spotted cDNA fragments encoding 288 mouse genes related to neurogenesis and neural stem cell differentiation, as well as notch signaling elements. Control sequences (PUC18, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), peptidylpropyl isomerase A (Ppia), and β-actin) were also included. These microarrays were employed to compare SVZ gene expression between WT and CNP-hEGFR mouse SVZ. Total RNA was isolated by Trizol (Invitrogen) and processed for microarray hybridization following the ma

ORGANISM(S): Mus musculus

SUBMITTER: Vittorio Gallo 

PROVIDER: E-GEOD-21913 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress