COP1 siRNA knockdown in human Hepatocellular carcinoma cells

ABSTRACT: Development of targeted therapeutics for hepatocellular carcinoma (HCC) remains a major challenge. We have previously demonstrated that constitutively photomorphogenic 1 (COP1), which regulates p53 activity by ubiquitination, is frequently overexpressed in human HCC. Here we examined whether molecular targeting of COP1 by small interfering (si) RNA can affect the course of HCC progression. COP1-1 was selected as the most effective target siRNAs in terms of growth inhibition and apoptotic induction in several HCC cell lines. Interestingly, the growth inhibition occurred both in HCC cells that retain wild-type p53 or express mutant p53 (Y220C or R249S). Next we have determined to investigate the molecular mechanisms underlying the phenotypic changes. Given the recent findings that COP1 funct

ORGANISM(S): Homo sapiens

SUBMITTER: Yun-Han Lee 

PROVIDER: E-GEOD-21955 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress