Transition from succinate to methanol growth in Methylobacterium Extorquens AM1

ABSTRACT: In order to provide information about the gene expression response that occurs when cells experience a change in carbon source, succinate limited chemostat cultures of Methylobacterium extorquens AM1 were grown to and maintained at an OD of ~0.63, transferred to flasks and methanol was added. Cells were harvested for RNA extraction at time: 0 min, 10 min, 30 min, 1 hr, 2 hr, 4 hr and 6 hr post transition. At 30 min, a no methanol addition sample was extracted as a carbon starvation control. These data were used in conjunction with flux, enzymatic and metabolite measurements to assess the changes in central metabolism during this transition. Abstract from manuscript: When organisms experience environmental change, how does their metabolic network reset and adapt to the new condition? Thi

ORGANISM(S): Methylobacterium extorquens AM1

SUBMITTER: Elizabeth Skovran 

PROVIDER: E-GEOD-22031 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress