Stable expression of intracellular Notch suppresses v-Src-induced transformation in avian neural cells

ABSTRACT: We reported that stable expression of constitutively active intra cellular Notch (ICN), in quail neuroretina (QNR) cells transformed by a conditional v-Src mutant (QNR/v-src cells), resulted in the suppression of their transformed properties. Acquisition of a normal phenotype coincided with a major switch in cell identity, as these undifferentiated QNR/v-src cells acquired characteristics of glial differentiation. Similar loss of transformation and gene reprogramming can be achieved in QNR/v-src cells, stably expressing the human CBF protein, RBP-Jk, whose activity was rendered ligand independent by fusion to the VP16 transactivator. These major phenotypic changed are correlated with a dominant interference with signaling effectors, regulating cell morphology and cytoskeleton organization.

ORGANISM(S): Gallus gallus

SUBMITTER: georges calothy 

PROVIDER: E-GEOD-22054 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress