Expanding the MicroRNA Targeting Code: A Novel Type of Site with Centered Pairing

ABSTRACT: We present “centered sites,” a class of microRNA target sites that lacks both perfect seed pairing and 3'-compensatory pairing and instead has 11–12 contiguous Watson–Crick pairs to the center of the microRNA. In elevated Mg2+, centered sites impart mRNA cleavage, but in cells, centered sites repress protein output without consequential Agronaute-catalyzed cleavage. Our study also identified novel extensively paired sites that are cleavage substrates in cultured cells and human brain. This expanded repertoire of cleavage targets and the identification of the centered site type help explain why central regions of many microRNAs are evolutionarily conserved. To study centered sites and identify miRNA cleavage targets, mRNA degradomes were sequenced from human brain and HeLa cells, and sma

ORGANISM(S): Homo sapiens

SUBMITTER: Jin-Wu Nam 

PROVIDER: E-GEOD-22068 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress