Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Changes in gene expression in inter-implantation sites by absence of Cbs

ABSTRACT: The change in gene expression on the 8th day of gestation was investigated using DNA microarrays. Uterine gene expression of interimplanted sites was analyzed in female mice. Female mice, C57BL/6jXOla129 genetic background were used for experiments. Mice, housed in sterile filter-top cages (3-4 per cage), were acclimatized in a room maintained at 24ºC with a 12-h light-dark fr four months. Two groups were established: a) The control group and b) homozygous Cbs deficient mice and sacrificed at the eight day post-coitum.

ORGANISM(S): Mus musculus

SUBMITTER: Jesus Osada

PROVIDER: E-GEOD-22189 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Cystathionine β-synthase deficiency causes infertility by impairing decidualization and gene expression networks in uterus implantation sites.

Nuño-Ayala Mario M Guillén Natalia N Arnal Carmen C Lou-Bonafonte José M JM de Martino Alba A García-de-Jalón Jose-Antonio JA Gascón Sonia S Osaba Lourdes L Osada Jesús J Navarro María-Angeles MA

Physiological genomics 20120522 14

Hyperhomocysteinemia has been reported in human reproduction as a risk factor for early pregnancy loss, preeclampsia, and congenital birth defects like spina bifida. Female infertility was also observed in cystathionine beta synthase-deficient mice (Cbs-KO) as an animal model for severe hyperhomocysteinemia. The aim for the present research was to elucidate the time-point of pregnancy loss and to pinpoint gene and cellular changes involved in the underlying pathological mechanism. By mating 90-d ...[more]

PMID: 22617046

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Project description:Recents studies in mice and humans demonstrated the relevance of H2S-synthesising enzymes (such as CTH, CBS and MPST) in adipose tissue physiology and preadipocyte differentiation into adipocytes. Here, we aimed to investigate the combined role of CTH, CBS and MPST in the preservation of adipocyte protein persulfidation and adipogenesis. Joint CTH, CBS and MPST gene knockdown was achieved treating fully human adipocytes with siRNAs against these transcripts (siRNA_MIX). Adipocyte protein persulfidation was analyzed by a mass spectrometry label-free quantitative approach coupled with a dimedone-switch method for protein labeling and purification. The proteomic analysis quantified 216 proteins with statistically different persulfidation levels in KD cells compared to control adipocytes. In fully differentiated adipocytes, CBS and MPST mRNA and protein levels were abundant, whereas CTH expression was very low. Of note, siRNA_MIX administration resulted in a significant decrease in CBS and MPST expression, without impacting on CTH. Dual CBS and MPST gene knockdown resulted in decreased expression of relevant genes for adipocyte biology, including adipogenesis, mitochondrial biogenesis and lipogenesis, but increased proinflammatory- and senescence-related genes, in parallel to a significant disruption in adipocyte protein persulfidation pattern. While among less persulfidated proteins, we identified several relevant proteins for adipocyte adipogenesis and function, among the most persulfidated, key mediators of adipocyte inflammation and dysfunction, but also some proteins that might have a positive role of adipogenesis were found. In conclusion, current study indicates that joint knockdown of CBS and MPST (but not CTH) in adipocytes impairs adipogenesis and promotes inflammation, possibly by disrupting the pattern of protein persulfidation in these cells, and suggesting that these enzymes were required for the functional maintenance of adipocytes.

Dataset Information

Changes in gene expression in inter-implantation sites by absence of Cbs

Publications

Cystathionine β-synthase deficiency causes infertility by impairing decidualization and gene expression networks in uterus implantation sites.

Similar Datasets

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