Zic3 ChIP-on-chip in mouse ES cells

ABSTRACT: The transcription factor Zic3 is required for maintenance of embryonic stem (ES) cell pluripotency (Lim LS et al, Mol Biol Cell. 2007;18:1348-1358). By genome-wide chromatin immunoprecipitation (ChIP-chip) in ES cells, we have identified 379 direct Zic3 targets, many of which are functionally associated with pluripotency, cell cycle, proliferation, oncogenesis and early embryogenesis. E14 cells were cultured to a density of 1 x 108 cells for each IP. Two biological replicates were performed per experiment. Cells were cross-linked for 10 minutes at room temperature with 1% (w/v) formaldehyde and the reaction subsequently quenched with 125mM glycine. Nuclear fractions were isolated and the DNA sheared to average lengths of 200-500 bp. For analysis on mouse promoter arrays, purified ChIP mat

ORGANISM(S): Mus musculus

SUBMITTER: Linda Lim 

PROVIDER: E-GEOD-22195 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress