Changes in EHEC virulence on exposure to epithelial cell secreted factors

ABSTRACT: EHEC is a food-borne pathogen that colonizes human GI tract and leads to infection. To understand the process of colonization and to decipher if any factors secreted by intestinal epithelial cells help EHEC during the infection process, we studied expression of EHEC virulence gene expression when exposed to intestinal epithelial cell conditioned medium. In our study, we observed that exposure to epithelial cell conditioned medium for 1 h and 3 h increases expression of 32 out of 41 EHEC LEE virulence genes. In addition, expression of the shiga toxin 1 (Stx1) gene is up-regulated at 1 h of exposure. Also, 17 genes encoded by prophage 933W, including those for Stx2, are also upregulated at both time-points. The increase in 933W prophage expression is mirrored by a 2.7-fold increase in intrac

ORGANISM(S): Escherichia coli O157:H7 str. EDL933

SUBMITTER: Tarun Bansal 

PROVIDER: E-GEOD-22285 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress