Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Changes in gene expression in implantation and inter-implantation sites by absence of Cbs

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE22187: Changes in gene expression in implantation sites by absence of Cbs GSE22189: Changes in gene expression in inter-implantation sites by absence of Cbs Refer to individual Series

ORGANISM(S): Mus musculus

SUBMITTER: Jesus Osada

PROVIDER: E-GEOD-22310 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Cystathionine β-synthase deficiency causes infertility by impairing decidualization and gene expression networks in uterus implantation sites.

Nuño-Ayala Mario M Guillén Natalia N Arnal Carmen C Lou-Bonafonte José M JM de Martino Alba A García-de-Jalón Jose-Antonio JA Gascón Sonia S Osaba Lourdes L Osada Jesús J Navarro María-Angeles MA

Physiological genomics 20120522 14

Hyperhomocysteinemia has been reported in human reproduction as a risk factor for early pregnancy loss, preeclampsia, and congenital birth defects like spina bifida. Female infertility was also observed in cystathionine beta synthase-deficient mice (Cbs-KO) as an animal model for severe hyperhomocysteinemia. The aim for the present research was to elucidate the time-point of pregnancy loss and to pinpoint gene and cellular changes involved in the underlying pathological mechanism. By mating 90-d ...[more]

PMID: 22617046

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Project description:In present, interspecies cloning and interspecies-pregnancy were studied for endangered species rescue. However, the low implantation and survival ratio, spontaneous abortion, and unknown reason embryos absorption are the common and difficult problems of interspecies-pregnancy. In order to discover the mechanism of interspecies-pregnant failure and find ways to overcome the xeon-pregnant obstacles, we chosen the rat embryos pregnant in mouse uterus as a interspecies-pregnancy model. Three groups were set, mouse embryos to mouse recipients (MM) as control group, rat embryos to mouse recipients (RM), and rat and mouse embryos to mouse recipients together (RMM) as experiment groups. The former studies showed that rat embryos live no longer than day 7 of mouse pregnancy (D7). Our results showed that rat embryos survived to D7, and still existed to day 9 of mouse pregnancy (D9) in RM group. Surprisingly, the rat embryos survived to day 13 of the mouse gestation (D13) in RMM group. Microarray analysis was used to detect the global-gene expression profile changes of the whole implantation sites among the three groups at D7 and D9. By this way, we screened out the genes promoting the implanted rat embryos development in a mouse uterus which helped the rat embryos survive to D13 in RMM group compared with RM group, and the genes hindering the rat embryos development in a mouse uterus which prevented rat embryos living longer than D7 in RM group and D13 in RMM group compared with MM group. These findings provide insights into the mechanism of interspecies pregnant failure and new idea for interspecies pregnant studies. Experiment Overall Design: microarray was use to screen the genes among the day 7 and day 9 implantation sites of rat embryos implantation sites in a mouse uterus between rat embryos transfer to mouse recipients and rat embryos transfer to mouse recipients with mouse embryos. The mouse day7 and day 9 embryos implantation sites were use as control. Experiment Overall Design: totally 6 samples were analyzed, each samples two replications (one of them had three replications).

Dataset Information

Changes in gene expression in implantation and inter-implantation sites by absence of Cbs

Publications

Cystathionine β-synthase deficiency causes infertility by impairing decidualization and gene expression networks in uterus implantation sites.

Similar Datasets

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