MRNA expression in mutS2 deletion mutant of Thermus thermophilus HB8

ABSTRACT: We analyzed the expression profile of mutS2 deletion mutant strain of Thermus thermophils HB8 during the exponential growth phase. Keywords: cell type comparison The mutant strains were precultured in 3 mL of rich medium for two times at 70°C and then subcultured to 1000 mL of rich medium. These cells were cultured at70°C for 300 min , and then cells were harvested from 50 ml of the culture. Total RNA were extracted from wild-type and mutS-lacking strain and used for the cDNA synthesis by SuperScript II (Invitrogen, Carlsbad, CA). The cDNA fragments were labeled with GeneChip DNA Labeling Reagent (Affymetrix, Santa Clara, CA), using terminal transferase according to the manufacturer’s instructions. The 3’-terminal-labeled cDNA was hybridized with a TTHB8401a520105F GeneChip (Affymetrix)

ORGANISM(S): Thermus thermophilus HB8

SUBMITTER: Kenji Fukui 

PROVIDER: E-GEOD-22349 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress