Rainbow trout erythrocytes stimulated with LPS and Poly(I:C)

ABSTRACT: The focus of this study was to investigate the response of cultured rainbow trout erythrocytes to lipopolysaccharide (LPS) and to the analog viral dsRNA Poly(I:C) using a salmonid-specific microarray platform enriched with immune-related genes. Although the transcriptomic response measured as the total number of differentially expressed genes was higher in LPS, the intensity response, in terms of genes with a FC>2, was greater in Poly(I:C) treated cells. These two treatments shared the regulation of some genes, but not alls followed the same pattern. Over representation of Gene Ontology functional categories showed us that Poly(I:C) treatment produced a immune response whereas LPS stimulation affect biological processes specially. Trout erythrocytes were isolated from blood by twice Ficoll

ORGANISM(S): Oncorhynchus mykiss

SUBMITTER: Davínia Capdevila 

PROVIDER: E-GEOD-22379 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress