Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ChIP-chip of Geobacter sulfurreducens PCA with antibody against RNAP and RpoD under various conditions

ABSTRACT: We applied a ChIP-chip approach to elucidate the binding profiles of RNAP and RpoD experimentally under different growth conditions. This technique localizes DNA fragments within DNA-protein complexes enriched by chromatin immunoprecipitation using high-density oligonucleotide tilling arrays. A 21 ChIP-chip study using immunoprecipitated DNA (IP-DNA) from three culture conditions for RNAP and four culture conditions for RpoD. The high-density oligonucleotide tiling arrays used consisted of 381,174 oligonucleotide probes spaced 20 bp apart (30-bp overlap between two probes) across the G. sulfurreducens genome (NimbleGen). Experiments were conducted as three bioliogical replicates (different cultures).

ORGANISM(S): Geobacter sulfurreducens PCA

SUBMITTER: Bernhard Palsson

PROVIDER: E-GEOD-22503 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Analysis of Geobacter sulfurreducens under multiple growth conditions

Project description:This SuperSeries is composed of the following subset Series: GSE22497: Transcriptome analysis of Geobacter sulfurreducens under multiple growth conditions GSE22503: ChIP-chip of Geobacter sulfurreducens PCA with antibody against RNAP and RpoD under various conditions GSE22511: Genome-wide transcription start site determination of Geobacter sulfurreducens under multiple growth conditions Refer to individual Series

2010-06-30 | E-GEOD-22512 | biostudies-arrayexpress

Geobacter sulfurreducens grown in various conditions: gene expression and RpoN ChIP-chip

Project description:This SuperSeries is composed of the following subset Series: GSE17834: Transcriptome analysis of Geobacter sulfurreducens grown with different nitrogen sources GSE17837: ChIP-chip of Geobacter sulfurreducens PCA with antibody against RpoN under various conditions. Refer to individual Series

2010-06-28 | E-GEOD-17838 | biostudies-arrayexpress

ChIP-chip of Geobacter sulfurreducens PCA with antibody against various sigma factors under various conditions

Project description:We applied a ChIP-chip approach to elucidate the binding profiles of sigma factors (RpH, RpoN and RpoS) experimentally under different growth conditions. This technique localizes DNA fragments within DNA-protein complexes enriched by chromatin immunoprecipitation using high-density oligonucleotide tilling arrays. A 10 ChIP-chip study using immunoprecipitated DNA (IP-DNA) from three culture condtions against three alternative sigma factors . The high-density oligonucleotide tiling arrays used were consisted of 381,174 oligonucleotide probes spaced 20 bp apart (30-bp overlap between two probes) across the G.sulfurreducens genome (NimbleGen). Experiments were conducted as three bioliogical replicates (different cultures).

2013-05-01 | E-GEOD-37778 | biostudies-arrayexpress

ChIP-chip of Geobacter sulfurreducens PCA with antibody against RpoN under various conditions.

Project description:We applied a ChIP-chip approach to elucidate the σN regulon experimentally under different growth conditions. This technique localizes DNA fragments within σN complexes enriched by chromatin immunoprecipitation using high-density oligonucleotide tilling arrays. A nine ChIP-chip study using immunoprecipitated DNA (IP-DNA) from three separate culture conditions. The high-density oligonucleotide tiling arrays used were consisted of 381,174 oligonucleotide probes spaced 20 bp apart (30-bp overlap between two probes) across the G.sulfurreducens genome (NimbleGen). Experiments were conducted as three bioliogical replicates (different cultures)

2010-06-30 | E-GEOD-17837 | biostudies-arrayexpress

Genome-wide transcription start site determination of Geobacter sulfurreducens under multiple growth conditions

Project description:Determine the transcription start sites at 1-bp resolution across the entire G. sulfurreducens genome in multiple growth conditions, including fresh water acetate-fumarate (FWAF) medium, nitrogen limitation and growth on electrode. Special capture of the 5' end of primary transcripts in G. sulfurreducens and sequencing with Illumina Genome Analyzer II.

2010-07-01 | E-GEOD-22511 | biostudies-arrayexpress

Transcriptome analysis of Geobacter sulfurreducens under multiple growth conditions

Project description:Investigation of comprehensive information about the transcripts (boundary, level, etc.) across the entire G. sulfurreducens genome in mulitple growth conditions, including in biofilm and on electrode. A five array study using total RNA recovered from two separate culture conditions of G. sulfurreducens. G. sulfurreducens were harvested one week after growth on electrode or to form biofilm. The high-density oligonucleotide tiling arrays used consisted of 381,174 oligonucleotide probes spaced 20 bp apart (30-bp overlap between two probes) across the G. sulfurreducens genome (NimbleGen). Experiments were conducted as two (electrode) or three (biofilm) biological replicates (different cultures).

2010-07-01 | E-GEOD-22497 | biostudies-arrayexpress

Transcriptome analysis of Geobacter sulfurreducens grown with different nitrogen sources

Project description:Investigation of comprehensive information about the expression level of RNA transcripts across the entire G.sulfurreducens genome with different nitrogen sources, including NH3, Gln, Asp and N2. A twelve chip study using total RNA recovered from four separate culture conditions of G.Sulfurreducens. G.Sulfurreducens PCA were harvested at mid-exponential phase. The high-density oligonucleotide tiling arrays used were consisted of 381,174 oligonucleotide probes spaced 20 bp apart (30-bp overlap between two probes) across the G.sulfurreducens genome (NimbleGen). Experiments were conducted as three bioliogical replicates (different cultures)

2010-06-30 | E-GEOD-17834 | biostudies-arrayexpress

ChIP-chip of MG1655 with antibody against E. coli RNAP beta subunit under various conditions

Project description:We integrated RNAP binding regions (RBRs) and mRNA transcript abundance to determine segments of contiguous transcription originating from promoter regions. To measure RBRs at a genome scale, we employed a ChIP-chip method to E. coli K-12 MG1655 grown in the presence or absence of rifampicin under multiple growth conditions using antibody against E. coli RNAP beta subunit. A twelve ChIP-chip study using immunoprecipitated DNA (IP-DNA) from four separate culture conditions with and/or without rifampicin treatment. The high-density oligonucleotide tiling arrays used were consisted of 371,034 oligonucleotide probes spaced 25 bp apart (25-bp overlap between two probes) across the E. coli genome (NimbleGen). Experiments were conducted as biological duplicates or triplicates (different cultures).

2009-11-03 | E-GEOD-15588 | biostudies-arrayexpress

ChIP-chip of E. coli K-12 MG1655 with antibody against RNA polymerase subunits, RpoB, RpoD, RpoS,RpoH, RpoN, FliA, and FecI

Project description:We investigate sigma factor binding regions for each sigma factor under various enviromental and/or genetic conditions. To measure sigma factor binding at a genome scale, we employed a ChIP-chip method to derivative strains of E. coli K-12 MG1655 wild type and its isogenic rpoS and rpoN knock-out strains under various conditions. A 45 ChIP-chip study under 4 separate culture conditions. The high-density oligonucleotide tiling arrays used were consisted of 371,034 oligonucleotide probes spaced 25 bp apart (25-bp overlap between two probes) across the E. coli genome.

2014-02-05 | E-GEOD-46541 | biostudies-arrayexpress

Analysis of the iron stimulon and fur regulon of Geobacter sulfurreducens [ChIP-chip]

Project description:Fur is a transcriptional regulator whose activity is dependent on Fe(II) concentrations. Chromatin immunoprecipitation (ChIP) coupled to microarray analysis allowed for the identification of Fur binding sites within the G. sulfurreducens genome. Wild type G. sulfurreducnes was cultured on fresh water media (harvested during log phase), and ChIP was used to identify Fur binding regions.

2014-03-10 | E-GEOD-54305 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data