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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Microarray analysis of gene expression in the Arabidopsis rlt1-1 rlt2-1 mutant

ABSTRACT: The Arabidopsis thaliana Imitation Switch (AtISWI) subfamily of ATP-dependent chromatin remodeling factors play essential roles in plant development. Here we show that the DDT domain transcription factor RINGLET (RLT) form a complex with AtISWI. RLT1 physically interacts with CHR11 in yeast cells. Single mutants rlt1-1 and rlt2-1 show no obvious developmental defects, while the rlt1-1 rlt2-1 double mutant phenocopies the AtISWI mutant chr11-1 chr17-1. In addtion, the RLT-ISWI complex selectively represses SEPALLATA, FRUITFULL and SUPPRESSOR OF OVEREXPRESSION OF CO 1, but not AGAMOUS and PISTILLATA in leaves. Leaves from 15-day-old seedlings of wild-type Col-0 and rlt1-1 rlt2-1 were used for RNA preparation.In the experiment data,3a3b refers to rlt1-1 rlt2-1.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Lin Xu

PROVIDER: E-GEOD-22584 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Imitation Switch chromatin remodeling factors and their interacting RINGLET proteins act together in controlling the plant vegetative phase in Arabidopsis.

Li Guang G Zhang Jiawei J Li Jiqin J Yang Zhongnan Z Huang Hai H Xu Lin L

The Plant journal : for cell and molecular biology 20120724 2

During their life cycle, flowering plants must experience a transition from vegetative to reproductive growth. Here, we report that double mutations in the Arabidopsis thaliana IMITATION SWITCH (AtISWI) genes, CHROMATIN REMODELING11 (CHR11) and CHR17, and the plant-specific DDT-domain containing genes, RINGLET1 (RLT1) and RLT2, resulted in plants with similar developmental defects, including the dramatically accelerated vegetative-to-reproductive transition. We demonstrated that AtISWI physicall ...[more]

PMID: 22694359

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Micro-array analysis of gene expression in the Arabidopsis chr11-1 chr17-1 mutant

Project description:Chromatin remodeling factors of the Imitation Switch (ISWI) family play important roles in epigenetic regulations of gene expression in yeast and animals, whereas their function in plants remains elusive. Here we report characterization of the Arabidopsis ISWI genes CHR11 and CHR17. Double mutant chr11 chr17 displayed a dramatically reduced plant size with early flowering. In addition, epidermis of the double mutant leaves showed cell characteristics seen only in floral organs. These phenotypes resemble, at least partially, those of the Polycomb mutants curly leaf (clf) and like heterochromatin protein1 (lhp1). Microarray analysis revealed that a number of targets of the Polycomb pathway were derepressed in chr11 chr17 leaves. Furthermore, triple mutants combining chr11 chr17 with clf-29 or lhp1-6 both greatly enhanced clf-29 and lhp1-6 phenotypes, respectively. All these results strongly suggest that the ISWI family genes in Arabidopsis may function in gene silencing via the Polycomb pathway Leaves from 15-day-old seedlings of wild-type Col-0 and chr11-1 chr17-1 were used for RNA preparation.In the experiment data,A refers to Col-0, and B refers to chr11-1 chr17-1.

2010-06-23 | E-GEOD-22159 | biostudies-arrayexpress

Microarray analysis of gene expression in the Arabidopsis rlt1-1 rlt2-1 mutant

Project description:The Arabidopsis thaliana Imitation Switch (AtISWI) subfamily of ATP-dependent chromatin remodeling factors play essential roles in plant development. Here we show that the DDT domain transcription factor RINGLET (RLT) form a complex with AtISWI. RLT1 physically interacts with CHR11 in yeast cells. Single mutants rlt1-1 and rlt2-1 show no obvious developmental defects, while the rlt1-1 rlt2-1 double mutant phenocopies the AtISWI mutant chr11-1 chr17-1. In addtion, the RLT-ISWI complex selectively represses SEPALLATA, FRUITFULL and SUPPRESSOR OF OVEREXPRESSION OF CO 1, but not AGAMOUS and PISTILLATA in leaves.

2010-07-03 | GSE22584 | GEO

Study of the molecular stability of patient-derived tumor xenograft (PDX) for human renal cell carcinoma (RCC) in nude mice during subsequent passages in mice

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Micro-array analysis of WT and AtISWI in callus formation

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2014-05-15 | E-GEOD-57145 | biostudies-arrayexpress

Sphere-Derived Multipotent Progenitor Cells Obtained From Human Oral Mucosa Are Enriched in Neural Crest Cells.

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2016-07-03 | E-GEOD-68636 | biostudies-arrayexpress

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2016-05-31 | E-GEOD-48467 | biostudies-arrayexpress

Reconstructing gene regulatory networks of tumorigenesis [genex]

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2016-05-13 | E-GEOD-72530 | biostudies-arrayexpress

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2010-03-22 | E-GEOD-20850 | biostudies-arrayexpress

microRNA156 resistant SQUAMOSA-PROMOTER-BINDING PROTEIN-LIKE13 (mSPL13) seedlings

Project description:The expression profiles were determined using Affymetrix ATH1 arrays. Comparisons among the Col-0, SPL13 and mSPL13 sample groups allow the identification of genes regulated by SPL13. Keywords: Potential upregulated and downregulated genes Arabidopsis 3 days seedling from three independent biological replicates of Col-0, SPL13 and mSPL13 samples, which contain three independent seed lots each, were analyzed.

2010-05-27 | E-GEOD-10414 | biostudies-arrayexpress

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