Genome-Wide Analysis of Estrogen Receptor-α DNA Binding and Tethering Mechanisms

ABSTRACT: The nuclear receptor, estrogen receptor alpha (ERα), controls the expression of hundreds of genes responsible for target cell phenotypic properties, but the relative importance of direct vs. tethering mechanisms of DNA binding has not been established. In this first report, we examine the genome-wide chromatin localization of an altered-specificity mutant ER with a DNA-binding domain deficient in binding to estrogen response element (ERE)-containing DNA (DBDmut ER) vs. wild type ERα. Using high-throughput sequencing of ER chromatin immunoprecipitations (ChIP-Seq) and mRNA transcriptional profiling, we show that direct ERE binding is required for most (75%) estrogen-dependent gene regulation and 90% of hormone-dependent recruitment of ER to genomic binding sites. De novo motif analysis of

ORGANISM(S): Homo sapiens

SUBMITTER: Joshua Stender 

PROVIDER: E-GEOD-22610 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress