Depletion of murine intestinal microbiota: effects on gut mucosa and epithelial gene expression

ABSTRACT: Inappropriate cross talk between mammals and their gut microbiota may trigger intestinal inflammation and drive extra-intestinal immune-mediated diseases. Studies with germ-free or gnotobiotic animals represent the gold standard for research on bacterial-host interaction but they are not readily accessible to the wide scientific community. We aimed at refining a protocol that in a robust manner would deplete murine intestinal microbiota and prove to have significant biologic validity. Previously published protocols for depleting mice of their intestinal microbiota by administering broad-spectrum antibiotics in drinking water were difficult to reproduce. We show that twice daily delivery of antibiotics by gavage depleted mice of their cultivable fecal microbiota and reduced the fecal b

ORGANISM(S): Mus musculus

SUBMITTER: Leonardo Meza-Zepeda 

PROVIDER: E-GEOD-22648 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress