Effect of LAE1 gene deletion on gene expression in Trichoderma

ABSTRACT: The mitosporic fungus Trichoderma reesei is an industrial producer of enzymes for degradation of lignocellulosic polysaccharides to soluble monomers that can be fermented to biofuels. The genes encoding these enzymes in T. reesei have recently been shown to be clustered in the genome. Here we will show that the expression of these genes is epigenetically controlled at the heterochromatin level by the protein methyltransferase LAE1. Deletion of lae1 led to a loss of expression of the major cellulase and hemicellulase encoding genes, and resulted in an inability to grow on cellulose. The cellulase null phenotype was also seen with known soluble inducers of enzymes active on cellulose. In contrast, introduction of a second copy of lae1 or its enhanced expression under a strong constitutive p

ORGANISM(S): Hypocrea jecorina

SUBMITTER: Christian Kubicek 

PROVIDER: E-GEOD-22687 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress