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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Affymetrix HG-U133A 2.0 Expression data

ABSTRACT: Expression .CEL files from Affymetrix HG-U133A 2.0 arrays using DNA from 14 human cell lines derived from metastasized melanoma Study of global gene expression differences in malignant melanoma cell lines with or without specific abberations in BRAF NRAS or CDKN2A. UKRV-Mel-16a was wrongly described as Ma-Mel-16a in Blöthner et al. 2005

ORGANISM(S): Homo sapiens

SUBMITTER: Andreas Gast

PROVIDER: E-GEOD-22787 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Effect of common B-RAF and N-RAS mutations on global gene expression in melanoma cell lines.

Bloethner Sandra S Chen Bowang B Hemminki Kari K Müller-Berghaus Jan J Ugurel Selma S Schadendorf Dirk D Kumar Rajiv R

Carcinogenesis 20050310 7

We studied global gene expression in three melanoma cell lines with the most common and potent V600E mutation in the B-RAF gene-four cell lines with a common Q61R mutation in the N-RAS gene and three cell lines with no mutations using human HG-U133A 2.0 micro-arrays with 22 277 transcripts. Data analysis using stringent criteria revealed several upregulated and downregulated genes in cell lines with B-RAF and N-RAS mutations compared with cell lines without mutations. We found 29 genes specifica ...[more]

PMID: 15760917

Publication: 1/2

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Affymetrix HG-U133A 2.0 Expression data from 5 human cell lines derived from metastasized melanoma

Project description:Study of global gene expression differences in malignant melanoma cell lines with or without specific abberations in BRAF NRAS or CDKN2A. Expression .CEL files from Affymetrix HG-U133A 2.0 arrays using total RNA from 5 human cell lines derived from metastasized melanoma

2014-10-25 | E-GEOD-62682 | biostudies-arrayexpress

Affymetrix HG-U133A 2.0 Expression data from 6 human cell lines derived from metastasized melanoma

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Affymetrix HG-U133A 2.0 Expression data

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Initiation of Invasion-Metastasis Cascade by DNp73

Project description:The experiment was designed to profile the transcriptome of human melanoma cell lines that differ in their delta ex2/3p73 expression. The differential gene expression of SK-Mel-29 stably overexpressing delta ex2/3p73-cDNA (SK-Mel-29.DNp73) was analyzed in comparison to parental cells with integrated empty vector . The DNp73-related gene expression signature was compared to the transcriptome of SK-Mel-103 and SK-Mel-147 cells with high endogenous DNp73 expression.

2014-05-01 | E-MEXP-3911 | biostudies-arrayexpress

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Project description:Mutations in the NRAS oncogene are present in up to 20% of melanoma. Here, we show that interferon alpha-inducible protein 6 (IFI6) is necessary for NRASQ61K-induced transformation and melanoma growth. IFI6 was transcriptionally upregulated by NRASQ61K, and knockdown of IFI6 resulted in DNA replication stress due to dysregulated DNA replication via E2F2. This stress consequentially inhibited cellular transformation and melanoma growth via senescence or apoptosis induction depending on the RB and p53 pathway status of the cells. NRAS-mutant melanoma were significantly more resistant to the cytotoxic effects of DNA replication stress-inducing drugs, and knockdown of IFI6 increased sensitivity to these drugs. Pharmacological inhibition of IFI6 expression by the MEK inhibitor trametinib, when combined with DNA replication stress-inducing drugs, blocked NRAS-mutant melanoma growth. Collectively, we demonstrate that IFI6, via E2F2 regulates DNA replication and melanoma development and growth, and this pathway can be pharmacologically targeted to inhibit NRAS-mutant melanoma. MEL-ST cells expressing either empty vector or mutant oncogenic RAS genes (HRAS v12, KRAS v12, NRAS Q61K) were used to isolate total RNA. The RNA was then used to perform gene expression analyses using the Illumina HumanHT-12 V4.0 Expression BeadChip array.

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