Transcription profiling of oral cavity samples from human squamous cell carcinoma patients

ABSTRACT: Metastasis via the lymphatics is a major risk factor in squamous cell carcinoma of the oral cavity (OSCC). We sought to determine whether the presence of metastasis in the regional lymph node could be predicted by a gene expression signature of the primary tumor. A total of 18 OSCCs were characterized for gene expression by hybridizing RNA to Affymetrix U133A gene chips. Genes with differential expression were identified using a permutation technique and verified by quantitative RT-PCR and immunohistochemistry. A predictive rule was built using a support vector machine, and the accuracy of the rule was evaluated using crossvalidation on the original data set and prediction of an independent set of four patients. Metastatic primary tumors could be differentiated from nonmetastatic primary t

ORGANISM(S): Homo sapiens

SUBMITTER: Rebekah O'Donnell 

PROVIDER: E-GEOD-2280 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress