Expression analysis of Shigella flexneri 2a strain 301

ABSTRACT: Investigation of whole genome gene expression to identify overlooked sRNAs and sORFs. Background The completion of numerous genome sequences has introduced an era of whole-genome study. However, many real genes, including small RNAs (sRNAs) and small ORFs (sORFs), are missed in genome annotation. In order to improve genome annotation, we sought to identify novel sRNAs and sORFs in Shigella, the principal etiologic agents of bacillary dysentery or shigellosis. Results Firstly, we identified 64 sRNAs in Shigella which is experimentally validated in other bacteria based on sequence conservation. Secondly, among possible approaches to search for sRNAs, we employed computer-based and tiling array based methods, followed by RT-PCR and northern blots. This allowed us to identify 12 sRNAs in Shige

ORGANISM(S): Shigella flexneri 2a str. 301

SUBMITTER: Junping Peng 

PROVIDER: E-GEOD-22800 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress