Expression data from lack of DNA topoisomerase I, DNA topoisomerase II and complete lack of both topoisomerases

ABSTRACT: Here we dissect the transcriptional response in S. cerevisiae cells lacking DNA topoisomerases. We use microarray technology coupled with a functional genomics approach and demonstrate intimate connections between topoisomerase dependency, promoter chromatin architecture and gene transcription. Our findings suggest that DNA topoisomerases I and II play a role for transcription initiation. We observe a genome wide reduction in mRNA levels and identify a distinct functional subset of the genome with particular requirements for topoisomerases. These genes are characterized by high transcriptional plasticity, they are chromatin regulated and distinguished by having an enrichment of a nucleosome at a critical position in the promoter region, suggesting that topoisomerases influence transcriptio

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jakob Pedersen 

PROVIDER: E-GEOD-22809 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress