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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Identification of differentially methylated regions between placentas and maternal blood cells on chromosome 18

ABSTRACT: Comparison of DNA methylation levels of chromosome 18 between placentas and blood cells of pregnant women Extracted DNA from 5 cases of placental tissues and 5 cases of maternal blood cell was subjected to MeDIP (methylated DNA immunoprecipitation) and hybridized to Affymetrix GeneChip® Human Tiling 2.0R Array sets. A control without immunoprecipitation (IP) was also processed in parallel with the immunoprecipitated DNA samples. The results were validated with bisulfite sequencing and mass spectrometry-based analysis

ORGANISM(S): Homo sapiens

SUBMITTER: Yuk Ming Lo

PROVIDER: E-GEOD-22837 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Systematic identification of placental epigenetic signatures for the noninvasive prenatal detection of Edwards syndrome.

Tsui Dana W Y DW Lam Y M Doris YM Lee Wing S WS Leung Tak Y TY Lau Tze K TK Lau Elizabeth T ET Tang Mary H Y MH Akolekar Ranjit R Nicolaides Kypros H KH Chiu Rossa W K RW Lo Y M Dennis YM Chim Stephen S C SS

PloS one 20101130 11

<h4>Background</h4>Noninvasive prenatal diagnosis of fetal aneuploidy by maternal plasma analysis is challenging owing to the low fractional and absolute concentrations of fetal DNA in maternal plasma. Previously, we demonstrated for the first time that fetal DNA in maternal plasma could be specifically targeted by epigenetic (DNA methylation) signatures in the placenta. By comparing one such methylated fetal epigenetic marker located on chromosome 21 with another fetal genetic marker located on ...[more]

PMID: 21152411

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