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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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MicroRNA expression in LKS hematopoietic progenitors vs. total bone marrow

ABSTRACT: Agilent chip was used to measure microRNA expression in the Lin- cKit+ Sca1+ bone marrow compartment and in total bone marrow. RNA was isolated by miRNeasy (Qiagen) from 1) Lin-cKit+Sca1+ cells, isolated from bone marrow using MACS column purification followed by fluorescent cell sorting, and 2) total Bone Marrow. Agilent microRNA microarray was run on these two samples.

ORGANISM(S): Mus musculus

SUBMITTER: Aadel Chaudhuri

PROVIDER: E-GEOD-22844 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MicroRNA-155 promotes autoimmune inflammation by enhancing inflammatory T cell development.

O'Connell Ryan M RM Kahn Daniel D Gibson William S J WS Round June L JL Scholz Rebecca L RL Chaudhuri Aadel A AA Kahn Melissa E ME Rao Dinesh S DS Baltimore David D

Immunity 20100930 4

Mammalian noncoding microRNAs (miRNAs) are a class of gene regulators that have been linked to immune system function. Here, we have investigated the role of miR-155 during an autoimmune inflammatory disease. Consistent with a positive role for miR-155 in mediating inflammatory responses, Mir155(-/-) mice were highly resistant to experimental autoimmune encephalomyelitis (EAE). miR-155 functions in the hematopoietic compartment to promote the development of inflammatory T cells including the T h ...[more]

PMID: 20888269

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microRNA expression in LKS hematopoietic progenitors vs. total bone marrow

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