OTX2 drives medulloblastoma proliferation via direct regulation of cell cycle genes and inhibits differentiation

ABSTRACT: The transcription factor OTX2 has been implicated as an oncogene in medulloblastoma, which is the most common malignant brain tumor in children. It is highly expressed in most medulloblastomas and amplified in a subset of them. The role of OTX2 in medulloblastoma and its downstream targets are unclear. Therefore, we generated D425 medulloblastoma cells in which we can silence endogenous OTX2 by inducible shRNA. Silencing of OTX2 strongly inhibited cell proliferation and resulted in a neuronal-like differentiation. Expression profiling of time courses after silencing showed a progressive change in gene expression for many cellular processes. Down regulated genes were highly enriched for cell cycle and visual perception genes, while up regulated genes were enriched for genes involved in deve

ORGANISM(S): Homo sapiens

SUBMITTER: Rogier Versteeg 

PROVIDER: E-GEOD-22875 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress