EWS siRNA knock-down in HeLa cell line
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: BELEN MINANA
PROVIDER: E-GEOD-22929 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
The Ewing sarcoma protein regulates DNA damage-induced alternative splicing.
Paronetto Maria Paola MP Miñana Belén B Valcárcel Juan J
Molecular cell 20110801 3
The Ewing sarcoma (EWS) protein is a member of the TET (TLS/EWS/TAF15) family of RNA- and DNA-binding proteins whose expression is altered in cancer. We report that EWS depletion results in alternative splicing changes of genes involved in DNA repair and genotoxic stress signaling, including ABL1, CHEK2, and MAP4K2. Chromatin and RNA crosslinking immunoprecipitation results indicate that EWS cotranscriptionally binds to its target RNAs. This association is reduced upon irradiation of cells with ...[more]
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