Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcript profiling of three genotypes by ultraviolet-B (UV-B) radiation

ABSTRACT: Due to ever increasing environmental deterioration it is likely that influx of solar UV-B radiation (280-320 nm) will increase further due to the depletion of stratospheric ozone. Given this fact it becomes essential that we better understand both rapid and adaptive responses of plants to UV-B imposed stress. Here we compare the transcriptmic responses of wild type Arabidopsis to that of Arabidopsis mutants impaired in flavonoid (TRANSPARENT TESTA4 [tt4] or sinapoyl-malate (sinapoylglucose accumulator 1 [sng1]) biosynthesis, to a short, 24h exposure to this photo-oxidative stress. In control experiments we subjected the same genotypes to 24h treatments of continuous light. Total 18 samples; number of biological replicates, n = 3 each; 2 different light treatments; three genotypes

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Miyako Kusano

PROVIDER: E-GEOD-22951 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Ouyang2014 - photomorphogenic UV-B signalling network This model is described in the article: Coordinated photomorphogenic UV-B signaling network captured by mathematical modeling. Ouyang X, Huang X, Jin X, Chen Z, Yang P, Ge H, Li S, Deng XW. Proc. Natl. Acad. Sci. U.S.A. 2014 Aug; 111(31): 11539-11544 Abstract: Long-wavelength and low-fluence UV-B light is an informational signal known to induce photomorphogenic development in plants. Using the model plant Arabidopsis thaliana, a variety of factors involved in UV-B-specific signaling have been experimentally characterized over the past decade, including the UV-B light receptor UV resistance locus 8; the positive regulators constitutive photomorphogenesis 1 and elongated hypocotyl 5; and the negative regulators cullin4, repressor of UV-B photomorphogenesis 1 (RUP1), and RUP2. Individual genetic and molecular studies have revealed that these proteins function in either positive or negative regulatory capacities for the sufficient and balanced transduction of photomorphogenic UV-B signal. Less is known, however, regarding how these signaling events are systematically linked. In our study, we use a systems biology approach to investigate the dynamic behaviors and correlations of multiple signaling components involved in Arabidopsis UV-B-induced photomorphogenesis. We define a mathematical representation of photomorphogenic UV-B signaling at a temporal scale. Supplemented with experimental validation, our computational modeling demonstrates the functional interaction that occurs among different protein complexes in early and prolonged response to photomorphogenic UV-B. This model is hosted on BioModels Database and identified by: BIOMD0000000545. To cite BioModels Database, please use: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

Project description:UV radiation (UV) alters secondary metabolism in the skin of Vitis vinifera L. berries, which may affect on the final composition of both, grapes and wines. We compared berry skin transcriptome and phenolic composition between Tempranillo berries grown in the presence or absence of solar UV in a mid-altitude Tempranillo vineyard. By analysing two different ripening degrees, expression of 121 genes was significantly altered. Functional enrichment identified that, principally, secondary metabolism-related transcripts were induced by UV, including VvFLS1, VvGT5 and VvGT6 flavonol biosynthetic genes induction. Concurrently, flavonol accumulation was the most evident impact of UV on the berry skin phenolic composition. Monoterpenoid biosynthetic transcripts were also up-regulated by UV, whereas induction of stilbenoid biosynthetic transcripts and stilbenes accumulation was probably induced by the joint action of UV and other condition under the UV-blocking filter, likely higher temperature. Among regulatory genes, VvMYBF1, VvMYB24 and three bHLH transcription factors were up-regulated by UV. Homologs to Arabidopsis UVR8-dependent UV-B-induced genes were also induced, including VvHY5-1, VvHY5-2 and VvRUP UV-B signalling genes. This suggests that the UV-B-specific signalling pathway is activated in the skin of grapes grown at low-medium altitudes. The biosynthesis and accumulation of UV-absorbing compounds that are appreciated for winemaking were almost specifically triggered, which indicates that viticultural practices increasing solar UV incidence may improve grape features important to wine production. A total of 12 samples were hybridized. Grape skin RNA from berries ripening under a UV-transmitting filter (FUV+) and a UV-blocking filter (FUV-) was compared. Berry skin of two different ripening stages was analysed on each UV treatment. All samples were harvested simultaneously and a NaCl series was used to select the ripening degree in a non-invasive way. Three biological replicates were analyzed for each sample.

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Transcript profiling of three genotypes by ultraviolet-B (UV-B) radiation

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