Effect of Medroxyprogesterone Acetate on Transcript Profile of Human Pregnant Myometrium

ABSTRACT: Context: Progesterone is important physiologically and therapeutically to maintain uterine quiescence during pregnancy. It acts in part through controlling myometrial gene expression. Objective: To use expression microarray and qRT-PCR validation to determine the changes in gene expression induced by prolonged exposure to a synthetic progestogen. Design: Myometrial explants, obtained at elective Caesarean section (n=9 patients), were maintained in culture, under 0.6g tension, for 3 days in the presence or absence of medroxyprogesterone acetate (MPA, 100nM). Expression array was performed using Illumina human-8 v3 beadchip arrays. Approximately 30% of differentially expressed transcripts were validated in biological replicates (n=10) using qRT-PCR. Results: Transcripts from 114 genes wer

ORGANISM(S): Homo sapiens

SUBMITTER: D. Stephen Charnock-Jones 

PROVIDER: E-GEOD-22961 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress