Transcription profiling of mouse C57BL/6 brain
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
SUBMITTER: Sridar Chittur
PROVIDER: E-GEOD-2297 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Similar Datasets
Project description:1 week and 8 week male and female brains from littermates of age matched C57BL/6 mice week used to study expression profiles. Affymetrix MOE430A Genechip was used Keywords: other
2005-11-28 | GSE2297 | GEO
Project description:In the present work endothelial function in the aorta and femoral artery assessed in vivo by magnetic resonance imaging (MRI) was characterized in male and female 8-, 14-, 22-, 28-, and 40-week-old E3L.CETP and C57BL/6J mice. Vascular nitric oxide (NO), eicosanoids and hydrogen peroxide (H2O2) production in the aorta, were measured by electron paramagnetic resonance spectroscopy (EPR), mass spectrometry (LC/MS) and fluoresence assay, respectively. Endothelial-specific protein plasma biomarkers and global alterations in plasma proteome were asssesed by targeted and non-targeted preotomics, respectively. In C57BL/6J endothelial dysfunction was observed in 40-week-old female and male mice as evidenced by impaired endothelium-dependent vasodilation induced by acetylcholine (Ach) in the aorta or by flow in the femoral artery (flow-mediated vasodilation, FMD). In E3L.CETP mice age-dependent endothelial dysfunction was accelerated and appeared in 14-22-week-old male and 22-28-week-old female mice. In 40 week-old E3L.CETP mice endothelial dysfunction was severe in both male and female mice and was more pronounced as compared with age-matched C57BL/6J mice. Despite severe endothelial dysfunction in 40 week-old mice E3L.CETP mice neither in the aortic roots nor in brachiocephalic artery atherosclerotic plaques were not detected. Interestingly, in the presence of NOS-inhibitor (L-NAME), FMD was inhibited in all experimental groups. However, effect of L-NAME on Ach–induced vasodilation in E3L.CETP mice, was blunted as compared with C57BL/6J mice, in particular in young E3L.CETP female mice. Furthermore, Ach–induced vasodilation in the aorta was inhibited by catalase, while H2O2 production was increased, in young female but not in male E3L.CETP mice. A switch from NO to H2O2-dependent vasodilation in young female E3L.CETP mice was associated with a blunted systemic inflammation and lower number of differentially expressed proteins (DEPs) in plasma than in young E3L.CETP male mice as compared with age-and sex-matched C57BL/6J mice. However, female and male 40-week-old E3L.CETP mice displayed similar number of DEPs in plasma vs respective sex-matched younger E3L.CETP mice. In the present work endothelial function in the aorta and femoral artery assessed in vivo by magnetic resonance imaging (MRI) was characterized in male and female 8-, 14-, 22-, 28-, and 40-week-old E3L.CETP and C57BL/6J mice. Vascular nitric oxide (NO), eicosanoids and hydrogen peroxide (H2O2) production in the aorta, were measured by electron paramagnetic resonance spectroscopy (EPR), mass spectrometry (LC/MS) and fluoresence assay, respectively. Endothelial-specific protein plasma biomarkers and global alterations in plasma proteome were asssesed by targeted and non-targeted preotomics, respectively. In C57BL/6J endothelial dysfunction was observed in 40-week-old female and male mice as evidenced by impaired endothelium-dependent vasodilation induced by acetylcholine (Ach) in the aorta or by flow in the femoral artery (flow-mediated vasodilation, FMD). In E3L.CETP mice age-dependent endothelial dysfunction was accelerated and appeared in 14-22-week-old male and 22-28-week-old female mice. In 40 week-old E3L.CETP mice endothelial dysfunction was severe in both male and female mice and was more pronounced as compared with age-matched C57BL/6J mice. Despite severe endothelial dysfunction in 40 week-old mice E3L.CETP mice neither in the aortic roots nor in brachiocephalic artery atherosclerotic plaques were not detected. Interestingly, in the presence of NOS-inhibitor (L-NAME), FMD was inhibited in all experimental groups. However, effect of L-NAME on Ach–induced vasodilation in E3L.CETP mice, was blunted as compared with C57BL/6J mice, in particular in young E3L.CETP female mice. Furthermore, Ach–induced vasodilation in the aorta was inhibited by catalase, while H2O2 production was increased, in young female but not in male E3L.CETP mice. A switch from NO to H2O2-dependent vasodilation in young female E3L.CETP mice was associated with a blunted systemic inflammation and lower number of differentially expressed proteins (DEPs) in plasma than in young E3L.CETP male mice as compared with age-and sex-matched C57BL/6J mice. However, female and male 40-week-old E3L.CETP mice displayed similar number of DEPs in plasma vs respective sex-matched younger E3L.CETP mice.
2025-06-30 | PXD029562 | Pride
Project description:IAV strains: California/4/09 (H1N1); Vietnam/1203/04 (H5N1-HALo)
Mice: six to eight week-old female C57BL/6 (Jackson Laboratory, Bar Harbor, ME) Dosing: 10^4 PFU per mouse; intranasal inoculation
Time points: 12h, 1, 2, 3, 4 days post infection
Control (mock): PBS
2022-08-08 | MSV000090095 | MassIVE
Project description:IAV strains: California/4/09 (H1N1)
Mice: six to eight week-old female C57BL/6 (Jackson Laboratory, Bar Harbor, ME)
Dosing: 10^4 PFU per mouse; intranasal inoculation
Time points: 12h, 1, 2, 3, 4 days post infection
Control (mock): PBS
2022-08-08 | MSV000090097 | MassIVE
Project description:IAV strains: California/4/09 (H1N1); Vietnam/1203/04 (H5N1-HALo)
Mice: six to eight week-old female C57BL/6 (Jackson Laboratory, Bar Harbor, ME)
Dosing: 10^4 PFU per mouse; intranasal inoculation
Time points: 12h, 1, 2, 3, 4 days post infection
Control (mock): PBS
2022-08-08 | MSV000090094 | MassIVE
Project description:IAV strains: Vietnam/1203/04 (H5N1-HALo)
Mice: six to eight week-old female C57BL/6 (Jackson Laboratory, Bar Harbor, ME)
Dosing: 10^4 PFU per mouse; intranasal inoculation
Time points: 12h, 1, 2, 3, 4 days post infection
Control (mock): PBS
2022-08-08 | MSV000090096 | MassIVE
Project description:Ventricular tissue of 8 week female PA mice (https://pubmed.ncbi.nlm.nih.gov/23648696/) and their female wild-type littermates were processed for phosphoproteomics
2023-09-28 | PXD043384 | Pride
Project description:Three groups of sex- and aged-matched 9-12 week old C57BL/6 wild type mice and gp91-/-phox mice were infected by i.v. injection of bacterial suspensions: Group 1 represents wild type C57BL/6 mice infected with virulent S. Typhimurium SL1344 grown in vitro. (Raw files: OTNCS_AGSL1344#3C#9C#10C-XX) Group 2 represents wild type C57BL/6 mice infected with virulent S. Typhimurium SL1344 grown in vivo for 72 h in the Group 1 C57BL/6 mice. (Raw files: OTNCS_AGSL1344#9F#10F-XX) Group 3 represents immunodeficient gp91-/-phox mice infected with virulent S. Typhimurium SL1344 grown in vitro. (Raw files: OTNCS_AGSL1344#11C#12C#13C-XX) And Input (Raw files: OTNCS_AGSL1344mixedAa-XX and OTNCS_AGSL1344mixedAb-XX)
2018-10-26 | PXD005855 | Pride
Project description:GL261-derived glioblastoma stem cells (GSCs) form aggressive tumors when implanted into the brains of C57BL/6 mice. We used snRNA-Seq to analyze GL261 and GL261-GSCs as well as tumor samples of 7 and 28 days of development.
2024-03-28 | GSE246154 | GEO
Project description:Cy3-labeled cDNA from brains of neonatal C57BL Cx43 null, Cx43 heterozygous and Cx32 null mice were compared among themselves and to Cy3-labeled cDNA from brains of neonatal C57BL wildtype mice through Cy5-labeled sample reference prepared at once for the entire experiment from aorta, brain, heart, kidney, liver, lung, ovary/testicles, spleen, and stomach - equal amounts from adult male and female C57BL mice.
2004-11-12 | GSE1954 | GEO