Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Alternatively Spliced Variants of Interleukin-4 Promote Inflammation Differentially

ABSTRACT: IL-4d2 is a natural splice variant of IL-4 which lacks the region encoded by the second exon. Numerous recent reports suggested that the expression levels of IL-4d2 change in various diseases, especially those with pulmonary involvement, but the effects of IL-4d2 on the lungs in vivo have never been studied. Replication-deficient adenovirus-mediated gene delivery of mouse IL-4d2 to mouse lungs in vivo was used, and the effects compared with similar adenoviral delivery of mouse IL-4 or with infection with a NULL viral construct. The objective was to determine whether full-length wild-type Interleukin-4 encoded by exons 1-4 (IL-4) and alternatively spliced variant econded by exons 1,3, and 4 (IL-4d2) differentially affect gene expression in the lungs in vivo. The results show that IL-4d2 and IL-4 affected global gene expression differentially. Keywords: Comparative analysis of gene delivery of alternative splice variants in vivo There were three mice overexpressing mouse IL-4 in their lungs, three mice overexpressing mouse IL-4d2 in their lungs, and three mice similarly infected with control AdV-NULL virus not encoding a cytokine.

ORGANISM(S): Mus musculus

SUBMITTER: Sergei Atamas

PROVIDER: E-GEOD-23016 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Alternatively spliced variants of interleukin-4 promote inflammation differentially.

Luzina Irina G IG Lockatell Virginia V Todd Nevins W NW Highsmith Kendrick K Keegan Achsah D AD Hasday Jeffrey D JD Atamas Sergei P SP

Journal of leukocyte biology 20110201 5

IL-4δ2 is a natural splice variant of IL-4 that lacks the region encoded by the second exon. Numerous reports have suggested that the expression levels of IL-4δ2 change in various diseases, especially those with pulmonary involvement, but the in vivo effects of this splice variant have never been studied. Replication-deficient, AdV-mediated gene delivery of mIL-4δ2 to mouse lungs in vivo was used, and the effects compared with similar adenoviral delivery of mIL-4 or with infection with a noncodi ...[more]

PMID: 21285395

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Dataset Information

Alternatively Spliced Variants of Interleukin-4 Promote Inflammation Differentially

Publications

Alternatively spliced variants of interleukin-4 promote inflammation differentially.

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