Differential mRNA accumulation in Arabidopsis lacking the exoribonuclease XRN4 indicates a preference of the enzyme for specific mRNA sequences and functional categories

ABSTRACT: One of the major players controlling RNA decay is the cytoplasmic 5’ to 3’ exoribonuclease, which is conserved among eukaryotic organisms. In Arabidopsis, the 5’ to 3’ exoribonuclease XRN4 is involved in disease resistance, the response to ethylene, RNAi and miRNA-mediated RNA decay. Curiously, XRN4 appears to display selectivity among its substrates because certain 3’ cleavage products formed by miRNA-mediated decay, such as from ARF10 mRNA, accumulate in the xrn4 mutant, whereas others, such as from AGO1, do not. To examine the nature of this selectivity, transcripts that differentially accumulate in xrn4 were identified by combining PARE and Affymetrix arrays. Certain functional categories, such as stamen-associated proteins and hydrolases, were over-represented among transcripts decrea

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Linda Rymarquis 

PROVIDER: E-GEOD-23027 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress