Chip-on-chip analysis of direct Nkx2.1 target genes in proliferating and differentiating epithelium in mouse lung development.

ABSTRACT: Nkx2.1 is a critical regulator of mammalian lung development. It is expressed in epithelial cells at the initiation of lung organogenesis, becoming progressively restricted during development to bronchiolar and alveolar type II epithelial cells. In humans, it is a common marker of carcinomas of lung and thyroid origin and is highly amplified in 10-15% of lung adenocarcinomas. Despite its key role in development and disease only a few genes directly regulated by Nkx2.1 are known. To identify direct Nkx2.1target genes and pathways controlled by this transcription factor in vivo we analyzed, by chromatin immuno-precipitation (chip)-on-chip method, Nkx2.1 binding to DNA cis-elements in developing mouse lung. We analyzed embryonic day E11.5 lungs, when Nkx2.1 expressing cells are undergoing pre

ORGANISM(S): Mus musculus

SUBMITTER: Maria Ramirez 

PROVIDER: E-GEOD-23043 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress