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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Murine hamatopoietic stem cell (HSC): wild type and Skp2 knockout

ABSTRACT: Transcriptional profiling of mouse HSC comparing wild type (WT) and Skp2 knockout Collected bone marrow (BM) cells from WT and Skp2 knockout mice, stained with HSC surface markers for FACS. Collected HSC and extracted total RNA then sent out for microarray analysis.

ORGANISM(S): Mus musculus

SUBMITTER: JING WANG

PROVIDER: E-GEOD-23064 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Skp2-macroH2A1-CDK8 axis orchestrates G2/M transition and tumorigenesis.

Xu Dazhi D Li Chien-Feng CF Zhang Xian X Gong Zhaohui Z Chan Chia-Hsin CH Lee Szu-Wei SW Jin Guoxiang G Rezaeian Abdol-Hossein AH Han Fei F Wang Jing J Yang Wei-Lei WL Feng Zi-Zhen ZZ Chen Wei W Wu Ching-Yuan CY Wang Ying-Jan YJ Chow Lu-Ping LP Zhu Xiao-Feng XF Zeng Yi-Xin YX Lin Hui-Kuan HK

Nature communications 20150330

Understanding the mechanism by which cell growth, migration, polyploidy, and tumorigenesis are regulated may provide important therapeutic strategies for cancer therapy. Here we identify the Skp2-macroH2A1 (mH2A1)-cyclin-dependent kinase 8 (CDK8) axis as a critical pathway for these processes, and deregulation of this pathway is associated with human breast cancer progression and patient survival outcome. We showed that mH2A1 is a new substrate of Skp2 SCF complex whose degradation by Skp2 promo ...[more]

PMID: 25818643

Publication: 1/2

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Murine hamatopoietic stem cell (HSC): wild type and Skp2 knockout

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Project description:rs04-04_skp2_cul4 - comparison_col0vsskp2 - Antisense and mutants of the SKP2 and CUL4 genes are compared to the Wt in order to identify the genes controlled by those SCF proteins. - Comparison Col0 versus skp2

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Project description:Murine hamatopoietic stem cell (HSC): wild type and Skp2 knockout

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A MYOD-SKP2 axis boosts tumorigenesis in fusion negative rhabdomyosarcoma by preventing differentiation through p57Kip2 targeting [RNA-seq]

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