Identification of microRNA targets in the mammalian inner ear using a comprehensive transcriptome and proteome integrated approach

ABSTRACT: We have employed a novel approach for the identification of functionally important microRNA (miRNA)-target interactions using integrated miRNA, transcriptome and proteome profiles with advanced in silico analysis. By looking at both the transcript and protein levels of expression, a thorough coverage of miRNA regulation was obtained. Microdissected auditory and vestibular sensory epithelia were used as the model system, thus being the first time such a comparison was carried out in a neuroepithelial system. Moreover, this is one of only a few studies employing proteome screening for the identification of miRNA targets. Notably, this approach can be employed for the study of other tissues and organs. We detected the expression of 157 miRNAs in the inner ear sensory epithelia, with 53 miRNAs

ORGANISM(S): Mus musculus

SUBMITTER: Tal Elkan-Miller 

PROVIDER: E-GEOD-23081 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress