Role of the Glc7 phosphatase regulatory subunit Ypi1 in progression through mitosis

ABSTRACT: Ypi1 is an essential regulator of the Saccharomyces cerevisiae Glc7 protein phosphatase. Although lack of Ypi1 results in a dramatic blockage in the G2/M cell cycle transition, with abnormally shaped large buds and short spindles, the molecular bases for this phenotype are still obscure. We report here that depletion of Ypi1 results in stabilization of the Pds1 securin, suggesting the activation of a G2/M checkpoint. Depletion of Ypi1 in cells deleted for MAD1/MAD2 or RAD9 still resulted in G2/M blockage, in spite that these cells lack key components of the spindle assembly and DNA damage checkpoints signaling, respectively. In contrast, deletion of SWE1, which encodes a protein kinase required for the morphogenesis checkpoint signaling, allowed passage through G2/M and recovery of normal

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Antonio Casamayor 

PROVIDER: E-GEOD-23134 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress