Generation of induced pluripotent stem cells in rabbits: potential experimental models for human regenerative medicine

ABSTRACT: Human induced pluripotent stem (iPS) cells have the potential to establish a new field of promising regenerative medicine. Therefore, the safety and the efficiency of iPS-derived cells must be tested rigorously using appropriate animal models before human trials can commence. Here, we report the establishment of rabbit iPS cells as the first human-type iPS cells generated from a small laboratory animal species. Using lentiviral vectors, four human reprogramming genes (c-MYC, KLF4, SOX2 and OCT3/4) were introduced successfully into adult rabbit liver and stomach cells. The resulting rabbit iPS cells closely resembled human iPS cells; they formed flattened colonies with sharp edges and proliferated indefinitely in the presence of bFGF. They expressed the endogenous pluripotency markers c-MYC

ORGANISM(S): Oryctolagus cuniculus

SUBMITTER: Arata Honda 

PROVIDER: E-GEOD-23190 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress