Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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L2_glutamate_receptor_expressing_neurons_tiling_array


ABSTRACT: modENCODE_submission_658 This submission comes from a modENCODE project of Robert Waterston. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: Transcript tiling array analysis EXPERIMENT TYPE: Transcript tiling array analysis. BIOLOGICAL SOURCE: Strain: NC1842 (engineered, target gene glr-1 tagged by 3xFlag::PAB-1); Tissue: Glutamate receptor expressing neurons (L2); Developmental Stage: larva mid-L2 20dC 22h post-L1; Genotype: unc-119 (ed1); [unc-119 (+); glr-1::3XFLAG::PAB-1]; Sex: mixed Male and Hermaphrodite population; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: Antibody EZview Red ANTI-FLAG® M2 Affinity Gel (target is FLAG); Strain NC1842 (engineered, target gene glr-1 tagged by 3xFlag::PAB-1); temperature 20; Developmental Stage larva mid-L2 20dC 22h post-L1; Tissue Glutamate receptor expressing neurons (L2)

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: DCC modENCODE 

PROVIDER: E-GEOD-23282 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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