Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of FACS sorted mouse calvarial osteroblasts at defined differentiation stages to identify genes highly associated with osteoblast differentiation

ABSTRACT: This study compares a comprehensive microarray analysis of unsorted mCOB cultures at two level of cell differentiation with cells that are more homogeneous for a level of cellular differentiation. It demonstrates the loss of information and in some cases misinformation that occurs when a heterogeneous population of cells are used for a microarray study. This work was sponsored by BMS.

ORGANISM(S): Mus musculus

SUBMITTER: Baikang Pei

PROVIDER: E-GEOD-2332 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Expression profile of osteoblast lineage at defined stages of differentiation.

Kalajzic Ivo I Staal Ada A Yang Wen-Pin WP Wu Yuli Y Johnson Susan E SE Feyen Jean H M JH Krueger Winfried W Maye Peter P Yu Fang F Zhao Yifang Y Kuo Lynn L Gupta Rishi R RR Achenie Luke E K LE Wang Hsin-Wei HW Shin Dong-Guk DG Rowe David W DW

The Journal of biological chemistry 20050415 26

The inherent heterogeneity of bone cells complicates the interpretation of microarray studies designed to identify genes highly associated with osteoblast differentiation. To overcome this problem, we have utilized Col1a1 promoter-green fluorescent protein transgenic mouse lines to isolate bone cells at distinct stages of osteoprogenitor maturation. Comparison of gene expression patterns from unsorted or isolated sorted bone cell populations at days 7 and 17 of calvarial cultures revealed an inc ...[more]

PMID: 15834136

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Dataset Information

Transcription profiling of FACS sorted mouse calvarial osteroblasts at defined differentiation stages to identify genes highly associated with osteoblast differentiation

Publications

Expression profile of osteoblast lineage at defined stages of differentiation.

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