B. thailandensis genes induced by subinhibitory concentration of glyphosate

ABSTRACT: Total transcript amplification (TTA) from single eukaryotic cells for transcriptome analysis is established, but TTA from a single prokaryotic cell presents additional challenges with much less starting material and lack of poly(A)-tails. We described, here, a novel method for single bacterium TTA, using a Burkholderia thailandensis model exposed to subinhibitory concentration of the antibacterial agent, glyphosate. Utilizing B. thialandensis microarray to assess the TTA method showed little gene bias (< 2 fold-change) and absence (~5-6%) when compared to the larger scale non-amplified RNA samples. B. thailandensis genes important to possibly recuperate and balance the intracellular amino acid pool were induced (or repressed) by the aromatic amino acid biosynthesis inhibitor, glyphosate. W

ORGANISM(S): Burkholderia thailandensis

SUBMITTER: Tung Hoang 

PROVIDER: E-GEOD-23419 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress